10bp Ladder
Concentration: 168ng/µl
Components
| Component | M1011 | M1012 |
| 10bp Ladder | 50ug | 5x50ug
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Description
10bp ladder is ideal for determining the size of double-stranded DNA from 80 to 300 base pairs in high percentage agarose or non-denaturing polyacrylamide gels. The ladder consists of 18 linear double-stranded fragments. It is a 10 bp step ladder, especially useful for determining 10 bp differences between DNA fragments. The 100bp and 200bp fragments are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 1 ul loading , all fragments except 100bp and 200bp are 8ng, the 100bp and 200bp fragments are 20ng. This ladder is pre-mixed with loading dye and is ready to use.
Recommended Loading
1 µl/Lane in non-denaturing polyacrylamide gel
3-5 µl/Lane in agarose gel
Concentration
Typical Bands, 20ng/µl
Other Bands, 8ng/µl
Recommended Electrophoresis Condition
1 µl/Lane, 20 cm 10% non-denaturing Polyacrylamide Gel, 1×TBE, 8 V/cm, 3h.
Contents (bp)
80,90,100,110,120,130,140,150,160,170,180,190,200,220,240,260,280,300.
Storage
Stable for 3 months at RT, for long-time storage, please store at -20 ̊C.
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Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.
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10bp Ladder DNA Marker M1011 (50μg)/M1012 (50μg×5) Images
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